Composite

Part:BBa_K5250021:Design

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-01)


pT7-like + GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 743
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 131
    Illegal AgeI site found at 592
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequences have been codon optimized.


Source

The sequence of the T7-like RNAP and promoter are taken from a Pseudomonas phage Pf-10 gene bank. The rest of the sequences are from the iGEM Parts Registry.

References